Benchmarking kun_peng with ZYMO D6311 sample

[humbleflowers]

I am running benchmarking on ZYMO D6311 containing 10 species in log proportion.
Theoretical Composition Based on Genomic DNA: Listeria monocytogenes - 89.1%, Pseudomonas aeruginosa - 8.9%, Bacillus subtilis - 0.89%, Saccharomyces cerevisiae - 0.89%, Escherichia coli - 0.089%, Salmonella enterica - 0.089%, Lactobacillus fermentum - 0.0089%, Enterococcus faecalis - 0.00089%, Cryptococcus neoformans - 0.00089%, and Staphylococcus aureus - 0.000089%
Command i ran was

kun-peng classify -p 30 --db /mnt/wwbixprd/kraken2_wwm_ont/k2-241224/ --output-dir test/ --chunk-dir chunk-test/ -S /mnt/wwbixprd/ont_zymo_samples/zym_D6311_r104.10gb.fastq.gz

I got following output

100.00  1       0       R       1       root
100.00  1       0       R1      131567    cellular organisms
100.00  1       0       D       2           Bacteria
100.00  1       0       K       1783272       Bacillati
100.00  1       0       P       1239            Bacillota
100.00  1       0       C       91061             Bacilli
100.00  1       0       O       1385                Bacillales
100.00  1       0       F       186820                Listeriaceae
100.00  1       1       G       1637                    Listeria

kun-peng identifes only one species at genus level. Is there a way to improve classification to identify all species. My database contains 25K organisms.
Thank you.

[eric9n]

To help investigate this issue, I would need to:

Check the database you're using:
Could you share where you downloaded the database from?
Or provide a link to the database you used?

Access the sample data:
Could you provide a link to the ZYMO D6311 sample data you used?
Or tell us where we can download the same sample?